1. Excise root and leaf segments, wash with 100mM potassium
phosphate buffer (pH7.0) for 3 times.
2. Immerse in the Gus substrate solution, 37C, dark, 12-24h
3. Rinse in phosphate buffer
4. Fix for 4h or longer
5. Rinse in the same buffer
6. Observe as whole specimens or as sections.
1M potassium phosphate buffer (pH7.0)
K2HPO4: 34.8g —>200ml
184.5ml K2HPO4+115.5ml KH2PO4—-> 300ml 1M potassium phosphate buffer
Gus substrate solution
Potassium phosphate buffer (pH7.0, 1M)
Store at -20C. Add 1mM x-gulc (MW521.8~~0.5mg/ml) freshly when use.
200mM sodium cacodylate, pH7.2