Immature embryo (12-15 days after pollination ) —->

Precultured on ND2 medium for 4 days at 26C in darkness —->

Immersed in the bacterial suspension for 20 minutes —->

Incubated on ND2-As medium for 3 days at 26C in darkness —->

Cultured on ND2CCH25 medium for 2 weeks for selection —->

Subcultured on ND2CCH50 medium for 4 weeks ( 2 generations ) for
selection —->

Resistant calli were put on 3M filter paper overnight for osmotic
treatment —->

Resistant calli were transferred to NN.1B2H medium for regeneration
—->

Regenerated seedlings were transferred to MS0 medium for further growth
—->

Plantlets about 10cm high were grown to maturity in greenhouse in soil