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Protocols

Molecular Biology

  • Agrobacterium mediated rice transformation
  • Arabidopsis transformation by vacuum infiltration
  • Bacterial mating with Bradyrhizobium
  • Biolistic-transformation of rice
  • Electroporation of E.Coli or Agrobacterium
  • Fluorimetric GUS-assay
  • Gus staining
  • Isolation of Bradyoxetin
  • Isolation of Soybean Seedling NolA Inducer
  • Liquid culture of Arabidopsis and chitin treatment
  • Liquid culture of Arabidopsis and chitin treatment
  • Microscopy and Immunolocalization of GS50 and GS52
  • Northern Blotting
  • Plant DNA isolation
  • Preparation of ultra-competent E. coli cells for transformation
  • Protocol for nodulation assay in pouches
  • RT-PCR

Others

  • Molecular weight of chitin oligomers and Nod factors
  • Protocol for Extraction of NOD Factors (LCOs) from Induced Bradyrhizobium Japonicum Cultures Using Resins
  • Recipe for B&D (Broughton and Dilworth 1971) nutrient solution for growing legumes in Leonard jars and pots

Transcriptomics

  • Midi RNA preparation using Trizol reagent
Recent News
  • Sam Yanders receives Mizzou Excellence Award for Undergraduate Research!

    May 4, 2023

    (read more)

  • Bruna Montes Luz receives GPC International Student Award

    April 27, 2023

    (read more)

  • Looking back on the sequencing of the soybean genome

    September 27, 2022

    (read more)

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