Protocols
  1. Protocol for nodulation assay in pouches
  2. Recipe for B&D (Broughton and Dilworth 1971) nutrient solution for growing legumes in Leonard jars and pots
  3. Protocol for Extraction of NOD Factors (LCOs) from Induced Bradyrhizobium Japonicum Cultures Using Resins
  4. RT-PCR
  5. Preparation of ultra-competent E. coli cells for transformation
  6. Plant DNA isolation
  7. Northern Blotting
  8. Microscopy and Immunolocalization of GS50 and GS52
  9. Liquid culture of Arabidopsis and chitin treatment
  10. Liquid culture of Arabidopsis and chitin treatment
  11. Isolation of Soybean Seedling NolA Inducer
  12. Isolation of Bradyoxetin
  13. Gus staining
  14. Fluorimetric GUS-assay
  15. Electroporation of E.Coli or Agrobacterium
  16. Biolistic-transformation of rice
  17. Bacterial mating with Bradyrhizobium
  18. Arabidopsis transformation by vacuum infiltration
  19. Midi RNA preparation using Trizol reagent
  20. Molecular weight of chitin oligomers and Nod factors
  21. Agrobacterium mediated rice transformation