201 Bond Life Sciences Center

1201 Rollins St

University of Missouri

Columbia, MO 65211

Email: ac9db@missouri.edu

 

Education

2018 – Present – PhD in Biochemistry (Molecular Biology)

Universidade Federal do Paraná (UFPR) – Curitiba, PR – Brazil

Current research focus: Nitrogen-fixing bacteria and plant interaction.

 

2016 – 2018: M. S. in Soil Science

Universidade Federal do Paraná (UFPR) – Curitiba, PR – Brazil

Thesis: Macroinvertebrate diversity in Amazonian Anthropic Soils

 

2009– 2015: B.S. – Agronomic Engineering

São Paulo State University (UNESP)– Registro, SP – Brazil

Final term project: Identification of earthworms used as biomarkers of soil contamination.

 

Publications

Morgante, P.G., Conrado, A.C. and Santiago, P.S., 2015. Molecular Identification of the Earthworm Amynthas Gracilis. Biophysical Journal, 108(2), p.395a.

Conrado, A.C., Arruda, H., Stanton, D.W., James, S.W., Kille, P., Brown, G., Silva, E., Dupont, L., Taheri, S., Morgan, A.J. and Simões, N., 2017. The complete mitochondrial DNA sequence of the pantropical earthworm Pontoscolex corethrurus (Rhinodrilidae, Clitellata): Mitogenome characterization and phylogenetic positioning. ZooKeys, (688), p.1.

James, S.W., Bartz, M.L., Stanton, D.W., Conrado, A.C., Dupont, L., Taheri, S., Silva, E.D., Cunha, L. and Brown, G.G., 2019. A neotype for Pontoscolex corethrurus (Müller, 1857)(Clitellata). Zootaxa, 4545(1), pp.124-132.

Nadolny, H., Santos, A., Demetrio, W., Ferreira, T., Maia, L.D.S., Conrado, A.C., Bartz, M., Garrastazu, M., Silva, E.D., Lavelle, P. and Baretta, D., 2020. Recommendations for assessing earthworm populations in Brazilian ecosystems. Pesquisa Agropecuária Brasileira, 55.

Demetrio, W.C., Conrado, A.C., Acioli, A.N., Casadei Ferreira, A., Bartz, M.L., James, S.W., da Silva, E., Maia, L.S., Martins, G.C., Macedo, R.S. and Stanton, D.W., 2021. A “Dirty” Footprint: Macroinvertebrate diversity in Amazonian Anthropic Soils. Global Change Biology.

 

 

Jordan Brungardt

Room 201

MU Bond Life Sciences Center
1201 Rollins Street
Columbia, Missouri 65211-7310

Phone: (316) 806-4631

 

Education

PhD, Plant Pathology; Kansas State University, Manhattan, KS May 2020

M.S., Biology; Wichita State University, Wichita, KS December 2014

B.S., Biology; Fort Hays State University, Hays, KS December 2009

 

Publications

Jordan Brungardt, Revathi Gonvind, and Harold N. Trick. (2019). A Simplified Method for Producing Laboratory Grade Recombinant TEV Protease from E. coli.

Jordan Brungardt, Timothy C. Todd, Thomas R. Oakley, and Harold N. Trick. 2019. Assessment of Soybean Cyst Nematode Bioassay Interference by Various Insecticides/miticides under Greenhouse Conditions. Plant Health Progress.

Bin Tian, Mónica Navia-Urrutia, Yueying Chen, Jordan Brungardt, and Harold N. Trick (2018) “Biolistic Transformation of Wheat” in Transgenic Plants: Methods and Protocols, Methods in Molecular Biology, Kumar et al. (ed.) Springer.

 

Research experience/employment

2014 – Present Graduate Research Assistant, Kansas State University

Mentor: Dr. Harold Trick

Introduction of a synthetic pathway in soybean for production of the SCN pheromone vanillic acid for increased SCN resistance

Production of recombinant antifungal peptides in soybean and wheat for increased pathogenic fungi resistance

2012 – 2014      Graduate Research Assistant, Wichita State University

Mentor: Dr. Bin Shuai

Thesis: Effects of mutations in GH3 genes on the interaction between the necrotrophic fungus Macrophomina phaseolina and its plant host Arabidopsis thaliana

The use of pasteurized microsclerotia of Macrophomina phaseolina as a source for priming systemic acquired resistance in Medicago truncatula.

2010 – 2012          Bio-Next, Wichita, Kansas

Position: Microbiologist, Quality control, Production assistant

2007 – 2010          Undergraduate Research Assistant, Fort Hays State University

Mentor: Dr. Brian Maricle

Effects of Ethylene on Root Tissue of Spartina anglica

Effects of Oil Well Brine on Plant Communities

 

Officer Positions

2016   President; KSU Plant Pathology Graduate Student Club

2016   Student Program Co-Chair; Society for In Vitro Biology

2015   Vice President; KSU Plant Pathology Graduate Student Club

 

Professional Presentations

2019   Biotech approaches for improved disease resistance in soybean and wheat; Stony Brook University, NY.  Biochemistry, Vitaly Citovsky lab

2017   Introduction of novel anti-fungal peptides into plants Presentation; K-State Entomology-Plant Pathology 3-Minute Thesis Competition

2016   Effects of transgenically expressed antimicrobial peptides in wheat on pathogenic fungi and bacteria Poster; Society for In Vitro Biology annual meeting

2016   Introduction of novel anti-fungal peptides into plants Presentation; K-State Entomology-Plant Pathology 3-Minute Thesis Competition

2015   Improved disease resistance in transgenic wheat using antimicrobial peptide genes

Poster, Society for In Vitro Biology annual meeting; K-State Graduate Research, Arts, and Discovery Forum

2014   Effects of mutations in GH3 genes on the interaction between the necrotrophic fungus Macrophomina phaseolina and its plant host Arabidopsis thaliana   Poster; Kansas Soybean Expo

2013   Interactions between the necrotrophic fungus Macrophomina phaseolina and Arabidopsis thaliana GH3 mutants (Presentation); Graduate Student Seminar Presentation; Department Seminar Wichita State University, Fall 2013

 

Professional Organizations

2013-Present   American Phytopathological Society

2015-Present   Society for In Vitro Biology

2015-Present   Golden Key International Honour Society

2007-2010        Botanical Society of America

 

Awards Received

2018   Christine Lee Shepard Memorial Fellowship

2017   Christine Lee Shepard Memorial Fellowship

2017   Richard Elmore Brown Graduate Student Teaching Award

2016   K-State SGA Student Club Travel Award

2015   Don C. Warren Genetic Fellowship

 

Certificates

2018   Plant Biosecurity in Theory and Practice, Kansas State University

 

Mentoring Activities

2016   Kendell Lolley, Kansas State University – Molecular techniques

2014   Tessa Schmidt, Wichita State University – Molecular techniques

2014   Katie Williams, Maize South High School – Molecular techniques

2013   Khai Huynh, Wichita State University – Molecular techniques

 

Courses taught

2015 – 2018 Graduate Teaching Assistant, Biotechnology; Kansas State University (2 semesters, 1 section per semester, 15-27 students per section)

Graduate Teaching Assistant, Introduction to Plant Pathology lab; Kansas State University (2 semesters, 1 section per semester, ~31 students per section)

2012 – 2014 Graduate Teaching Assistant, Genetics lab; Wichita State University (2 semesters; 5 sections total, ~25 students per section)

Graduate Teaching Assistant, Introduction to Microbiology lab; Wichita State University (1 semester, 2 sections, 22 students total)

2009               Teaching Assistant, Introduction to Botany lab; Fort Hays State University (2 lab sessions, 15 students)

 

 

 

Roseli Wassem

Division of Plant Sciences

201 Christopher S. Bond Life Sciences Center

1201 E. Rollins Street

University of Missouri-Columbia

Columbia MO 65211

Email: rwbdt@missouri.edu

Lab phone: (573) 884 4799

 

Education:

1991-1994 – Biology

Federal University of Paraná, Brazil

1995-2000 – Ph.D

Federal University of Paraná, Brazil

Thesis: Transcriptional regulation of the nifA gene of Herbasirillum seropedicae

 

Current research focus: Plant-microbial interaction

 

Professional Experience:

August 2002 – Associate Professor – Federal University of Paraná

March 2006- April 2007 Postdoctoral fellow at University of Geneva.

 

Publications:

Modulation of defence and iron homeostasis genes in rice roots by the diazotrophic endophyte Herbaspirillum seropedicae.

Brusamarello-Santos LCC, Alberton D, Valdameri G, Camilios-Neto D, Covre R, Lopes KP, Zibetti Tadra-Sfeir M, Faoro H, Adele Monteiro R, Barbosa-Silva A, John Broughton W, Oliveira Pedrosa F, Wassem R, Souza EM.

Sci Rep. 2019 Jul 22;9(1):10573. doi: 10.1038/s41598-019-45866-w.

 

Unveiling the Diversity of Immunoglobulin Heavy Constant Gamma (IGHG) Gene Segments in Brazilian Populations Reveals 28 Novel Alleles and Evidence of Gene Conversion and Natural Selection.

Calonga-Solís V, Malheiros D, Beltrame MH, Vargas LB, Dourado RM, Issler HC, Wassem R, Petzl-Erler ML, Augusto DG.

Front Immunol. 2019 Jun 4;10:1161. doi: 10.3389/fimmu.2019.01161. eCollection 2019.

 

A NodD-like protein activates transcription of genes involved with naringenin degradation in a flavonoid-dependent manner in Herbaspirillum seropedicae.

Wassem R, Marin AM, Daddaoua A, Monteiro RA, Chubatsu LS, Ramos JL, Deakin WJ, Broughton WJ, Pedrosa FO, Souza EM.

Environ Microbiol. 2017 Mar;19(3):1030-1040. doi: 10.1111/1462-2920.13604. Epub 2017 Feb 10.

 

Wheat colonization by an Azospirillum brasilense ammonium-excreting strain reveals upregulation of nitrogenase and superior plant growth promotion.  SANTOS, K. F. D. N. ; MOURE, V. R. ; HAUER, V. ; SANTOS, A. R. S ; DONATTI, L. ; GALVÃO, C. W. ; Pedrosa, F. O. ; SOUZA, E. M. ; Wassem, R. ; STEFFENS, M. B. R. . PLANT AND SOIL, v. 415, p. 245-255, 2017.

The NtrY-NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1.

Bonato P, Alves LR, Osaki JH, Rigo LU, Pedrosa FO, Souza EM, Zhang N, Schumacher J, Buck M, Wassem R, Chubatsu LS.

FEBS J. 2016 Nov;283(21):3919-3930. doi: 10.1111/febs.13897. Epub 2016 Oct 5.

 

RNA-seq analyses reveal insights into the function of respiratory nitrate reductase of the diazotroph Herbaspirillum seropedicae.

Bonato P, Batista MB, Camilios-Neto D, Pankievicz VC, Tadra-Sfeir MZ, Monteiro RA, Pedrosa FO, Souza EM, Chubatsu LS, Wassem R, Rigo LU.

Environ Microbiol. 2016 Sep;18(8):2677-88. doi: 10.1111/1462-2920.13422. Epub 2016 Jul 12.

 

Genetic and functional characterization of a novel meta-pathway for degradation of naringenin in Herbaspirillum seropedicae SmR1.

Maria Marin A, de la Torre J, Ricardo Marques Oliveira A, Barison A, Satie Chubatsu L, Adele Monteiro R, de Oliveira Pedrosa F, Maltempi de Souza E, Wassem R, Duque E, Ramos JL.

Environ Microbiol. 2016 Dec;18(12):4653-4661. doi: 10.1111/1462-2920.13313. Epub 2016 Apr 30.

 

Genome wide transcriptional profiling of Herbaspirillum seropedicae SmR1 grown in the presence of naringenin.

Tadra-Sfeir MZ, Faoro H, Camilios-Neto D, Brusamarello-Santos L, Balsanelli E, Weiss V, Baura VA, Wassem R, Cruz LM, De Oliveira Pedrosa F, Souza EM, Monteiro RA.

Front Microbiol. 2015 May 21;6:491. doi: 10.3389/fmicb.2015.00491. eCollection 2015.

 

Enhanced oxygen consumption in Herbaspirillum seropedicae fnr mutants leads to increased NifA mediated transcriptional activation.

Batista MB, Wassem R, Pedrosa Fde O, de Souza EM, Dixon R, Monteiro RA.

BMC Microbiol. 2015 May 7;15:95. doi: 10.1186/s12866-015-0432-6.

 

Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes.

Camilios-Neto D, Bonato P, Wassem R, Tadra-Sfeir MZ, Brusamarello-Santos LC, Valdameri G, Donatti L, Faoro H, Weiss VA, Chubatsu LS, Pedrosa FO, Souza EM.

BMC Genomics. 2014 May 16;15:378. doi: 10.1186/1471-2164-15-378.

 

The Herbaspirillum seropedicae SmR1 Fnr orthologs controls the cytochrome composition of the electron transport chain.

Batista MB, Sfeir MZ, Faoro H, Wassem R, Steffens MB, Pedrosa FO, Souza EM, Dixon R, Monteiro RA.

Sci Rep. 2013;3:2544. doi: 10.1038/srep02544.

 

Naringenin degradation by the endophytic diazotroph Herbaspirillum seropedicae SmR1.

Marin AM, Souza EM, Pedrosa FO, Souza LM, Sassaki GL, Baura VA, Yates MG, Wassem R, Monteiro RA.

Microbiology. 2013 Jan;159(Pt 1):167-75. doi: 10.1099/mic.0.061135-0. Epub 2012 Nov 1.

 

Draft genome sequence of Herbaspirillum lusitanum P6-12, an endophyte isolated from root nodules of Phaseolus vulgaris.

Weiss VA, Faoro H, Tadra-Sfeir MZ, Raittz RT, de Souza EM, Monteiro RA, Cardoso RL, Wassem R, Chubatsu LS, Huergo LF, Müller-Santos M, Steffens MB, Rigo LU, Pedrosa Fde O, Cruz LM.

J Bacteriol. 2012 Aug;194(15):4136-7. doi: 10.1128/JB.00657-12.

 

The type III secretion system is necessary for the development of a pathogenic and endophytic interaction between Herbaspirillum rubrisubalbicans and Poaceae.

Schmidt MA, Balsanelli E, Faoro H, Cruz LM, Wassem R, de Baura VA, Weiss V, Yates MG, Madeira HM, Pereira-Ferrari L, Fungaro MH, de Paula FM, Pereira LF, Vieira LG, Olivares FL, Pedrosa FO, de Souza EM, Monteiro RA.

BMC Microbiol. 2012 Jun 6;12:98. doi: 10.1186/1471-2180-12-98.

 

Genomic comparison of the endophyte Herbaspirillum seropedicae SmR1 and the phytopathogen Herbaspirillum rubrisubalbicans M1 by suppressive subtractive hybridization and partial genome sequencing.

Monteiro RA, Balsanelli E, Tuleski T, Faoro H, Cruz LM, Wassem R, de Baura VA, Tadra-Sfeir MZ, Weiss V, DaRocha WD, Muller-Santos M, Chubatsu LS, Huergo LF, Pedrosa FO, de Souza EM.

FEMS Microbiol Ecol. 2012 May;80(2):441-51. doi: 10.1111/j.1574-6941.2012.01309.x. Epub 2012 Feb 13.

 

CD80 and CD86 polymorphisms in populations of various ancestries: 5 new CD80 promoter alleles.

Beltrame MH, Pincerati MR, Dalla-Costa R, Wassem R, Köhler KF, Chautard-Freire-Maia EA, Tsuneto LT, Petzl-Erler ML.

Hum Immunol. 2012 Jan;73(1):111-7. doi: 10.1016/j.humimm.2011.10.018. Epub 2011 Oct 23.

 

Herbaspirillum-plant interactions: microscopical, histological and molecular aspects. MONTEIRO, Rose Adele ; Balsanelli, Eduardo ; WASSEM, Roseli ; Marin, Anelis M. ; Brusamarello-Santos, Liziane C. C. ; Schmidt, Maria Augusta ; Tadra-Sfeir, Michelle Z. ; Pankievicz, Vania C. S. ; Cruz, Leonardo M. ; Chubatsu, Leda S. ; Pedrosa, Fabio O. ; Souza, Emanuel M. .  Plant and Soil (Print), v. x, p. online first, 2012.

 

Differential gene expression of rice roots inoculated with the diazotrophHerbaspirillum seropedicae. Brusamarello-Santos, Liziane C. C. ; PACHECO, F. ; AL Janabi, S.M.M. ; MONTEIRO, Rose Adele ; CRUZ, Leonardo Magalhães ; BAURA, Valter Antonio de ; PEDROSA, Fabio de Oliveira ; SOUZA, Emanuel Maltempi de ; WASSEM, Roseli .  Plant and Soil (Print), v. 356, p. 115-123, 2012.

 

The type III secretion system is necessary for the development of a pathogenic and endophytic interaction between Herbaspirillum rubrisubalbicans and Poaceae.  Schmidt, Maria A ; Balsanelli, Eduardo ; FAORO, Helisson ; Cruz, Leonardo M ; WASSEM, Roseli ; de Baura, Valter A ; Weiss, Vinicius ; Yates, Marshall G ; Madeira, Humberto M F ; Pereira-Ferrari, Lilian ; Fungaro, Maria H P ; de Paula, Francine M ; Pereira, Luiz F P ; Vieira, Luiz G E ; Olivares, F bio L ; Pedrosa, F bio O ; de Souza, Emanuel M ; Monteiro, Rose A . BMC Microbiology (Online), v. 12, p. 98, 2012.

 

Nitrogen fixation control in Herbaspirillum seropedicae. CHUBATSU, Leda Satie ; MONTEIRO, Rose Adele ; Souza, Emanuel Maltempi ; OLIVEIRA, M. A. S. ; YATES, Marshall Geoffrey ; WASSEM, Roseli ; Bonatto, A.C. ; Huergo, Luciano Fernandes ; STEFFENS, Maria Berenice Reynaud ; RIGO, Liu Un ; PEDROSA, Fabio de Oliveira .  Plant and Soil (Print), v. 356, p. 197-207, 2012.

 

Genome of Herbaspirillum seropedicae strain SmR1, a specialized diazotrophic endophyte of tropical grasses.

Pedrosa FO, Monteiro RA, Wassem R, Cruz LM, Ayub RA, Colauto NB, Fernandez MA, Fungaro MH, Grisard EC, Hungria M, Madeira HM, Nodari RO, Osaku CA, Petzl-Erler ML, Terenzi H, Vieira LG, Steffens MB, Weiss VA, Pereira LF, Almeida MI, Alves LR, Marin A, Araujo LM, Balsanelli E, Baura VA, Chubatsu LS, Faoro H, Favetti A, Friedermann G, Glienke C, Karp S, Kava-Cordeiro V, Raittz RT, Ramos HJ, Ribeiro EM, Rigo LU, Rocha SN, Schwab S, Silva AG, Souza EM, Tadra-Sfeir MZ, Torres RA, Dabul AN, Soares MA, Gasques LS, Gimenes CC, Valle JS, Ciferri RR, Correa LC, Murace NK, Pamphile JA, Patussi EV, Prioli AJ, Prioli SM, Rocha CL, Arantes OM, Furlaneto MC, Godoy LP, Oliveira CE, Satori D, Vilas-Boas LA, Watanabe MA, Dambros BP, Guerra MP, Mathioni SM, Santos KL, Steindel M, Vernal J, Barcellos FG, Campo RJ, Chueire LM, Nicolás MF, Pereira-Ferrari L, Silva JL, Gioppo NM, Margarido VP, Menck-Soares MA, Pinto FG, Simão Rde C, Takahashi EK, Yates MG, Souza EM.

PLoS Genet. 2011 May;7(5):e1002064. doi: 10.1371/journal.pgen.1002064. Epub 2011 May 12.

 

Naringenin regulates expression of genes involved in cell wall synthesis in Herbaspirillum seropedicae.

Tadra-Sfeir MZ, Souza EM, Faoro H, Müller-Santos M, Baura VA, Tuleski TR, Rigo LU, Yates MG, Wassem R, Pedrosa FO, Monteiro RA.

Appl Environ Microbiol. 2011 Mar;77(6):2180-3. doi: 10.1128/AEM.02071-10. Epub 2011 Jan 21.

 

Evidence for the endophytic colonization of Phaseolus vulgaris (common bean) roots by the diazotroph Herbaspirillum seropedicae.

Schmidt MA, Souza EM, Baura V, Wassem R, Yates MG, Pedrosa FO, Monteiro RA.

Braz J Med Biol Res. 2011 Mar;44(3):182-5. Epub 2011 Jan 14.

Lijuan Zhou- Postdoctoral Research Associate

Contact information

202 Christopher S. Bond Life Sciences Center

1201 E. Rollins Street

University of Missouri

Columbia, MO 65211

Email: zhoulij@missouri.edu

Lab : (573) 884 4799

Education

Sep 2010 ~ Jan 2017

Ph.D in genetics

Shanghai Institute of Plant Physiology and Ecology

Chinese Academy of Sciences, Shanghai, China

Sep 2006 ~ Jul 2010

B.S. in Biotechnology

College of Marine Life Sciences

Ocean University of China, Qingdao, China

Professional Experience

May 2019 ~ present

Postdoctoral associate

Legume-Microbe Interactions Laboratory

University of Missouri, Columbia, MO, USA

Jul 2017 ~ Dec 2018

Postdoctoral associate

Molecular Genetics and Soybean Genomics Laboratory

University of Missouri, Columbia, MO, USA

Publications

Su-Hui Chen*, Li-Juan Zhou*, Ping Xu, and Hong-Wei Xue. SPOC domain-containing protein Leaf inclination 3 interacts with LIP1 to regulate rice leaf inclination through auxin signaling. PLOS Genetics 2018, doi:10.1371/journal.pgen.1007829.

Heng Ye, Manish Roorkiwal, Babu Valliyodan, Lijuan Zhou, Pengyin Chen, Rajeev K Varshney, and Henry T Nguyen. Genetic diversity of root system architecture in response to drought stress in grain legumes. Journal of Experimental Botany 2018, 69: 3267–3277.

Li-Juan Zhou, Lang-Tao Xiao, and Hong-Wei Xue. Dynamic cytology and transcriptional regulation of rice lamina joint development. Plant Physiology 2017, 174: 1728-1746.

Yajin Ye*, Lijuan Zhou*, Xue Liu, Hao Liu, Deqiang Li, Minjie Cao, Haifeng Chen, Lin Xu, Jiankang Zhu, and Yang Zhao. A novel chemical inhibitor of ABA signaling targets all ABA receptors. Plant Physiology 2017, 173: 2356-2369.

Kallie Jones
Email: KDJM5Q@mail.missouri.edu
Cell: 573-777-0657

Education

University of Missouri- Columbia
Bachelor of Science in Plant Sciences
Anticipated Graduation Date: May 2022
Awards: Bright Flight Scholar, Chancellor’s Award Recipient, CAFNR Scholarship Recipient, Don Day Agribusiness and Bioscience Award Recipient, MFA Foundation Scholarship Recipient, Katahdin Hair Sheep International Scholarship Recipient, Prenger Foods Scholarship recipient, Missouri Seal of Biliteracy Holder

Experience

Plant-Microbe Interactions in Laboratory of Dr. Gary Stacey, Oct 2018- Present
Undergraduate Research Assistant
Laboratory Techniques: preparing media, growing plants such as Seteria viridis and Arabidopsis, growing endophytic bacteria, plant inoculations, measuring root parameters with WinRHIZO software, DNA extraction, bacteria transformation with Plasmid DNA
Laboratory Equipment: air displacement pipettes, centrifuge, autoclave, gel electrophoresis, shaking incubator, incubator, spectrophotometer

EAG Labs, Columbia Missouri, June 2018- Present
Operations Support Technician
Laboratory Techniques: Good Laboratory Practices
Laboratory Equipment: Robot Coupe 10, 23, and 45, Hammermill

Presentations

Missouri Life Sciences Week 2019
Essential Genes for Beneficial Bacteria Association Identifies by Transposon Sequencing

Honors

Accepted into the FRIPS program (Freshman Research in Plant Sciences)

Contact information

Simoes de Araujo, Jean Luiz

203 Life Science Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: jstkz@missouri.edu

jean.araujo@embrapa.br

Phone: (573) 239 6045

 

Education:

1989-1994 – Agronomy

Federal Rural University of Rio de Janeiro, Brazil

 

1995-1997 – M.SC – Soil Microbiology

Federal Rural University of Rio de Janeiro, Brazil

 

January 1998 March 2002 – Ph.D  – Genetic and Molecular Biology

Federal University of Rio de Janeiro, Brazil

Thesis topic: Plant response to heat stress and Isolation of Heat Shock Proteins from cowpea.

 

Current research focus: Molecular Aspects of Plant Microbe Interaction: Biological Nitrogen Fixation and Plant Growth Promotion.

Research line: Associations between Plant Growth Promotion Bacteria (PGPRs) and grass.

 

Professional Experience:

March 2002 – August 2012 Postdoctoral Research, Biochemistry and Molecular Biology

Federal University of Rio de Janeiro, Brazil

Research topic: Sequencing and annotation of Plant Growth Promotion Bacteria Genomes.

 

August 2012 –

Researcher in the National Agrobiology Research Center from Embrapa (Brazilian Agricultural Research Corporation) https://www.embrapa.br/en/agrobiologia

Address to access complete CV: http://lattes.cnpq.br/4344808401973010

 

CNPq Research Productivity Scholarship – Level 2

Advisor professor in the Plant Biotechnology Post Graduate Course, Federal University of Rio de Janeiro, Brazil

 

 

Publications:

 

1. DA SILVA, H.A.P.; CAETANO, V.S.; VILLARINHO-PESSOA, D.D.; PACHECO, R.S.; SIMOES-ARAUJO, J.L. Molecular and biochemical changes of aging-induced nodules senescence in common bean. Symbiosis, 2019 (In press).

 

2. ZILLI, J.E.; ALVES, B.J.R. ROUWS, L.F.M.; SIMOES-ARAUJO, J.L.; et al. The importance of denitrification performed by nitrogen-fixing bacteria used as inoculants in South America. Plant and Soil, 2019, Special Issue S78 – Microorganisms in Agriculture (In press)

 

3. DA SILVA, P.R.A.; VIDAL, M.S.; SOARES, C.P.; POLESE, V.; TADRA-SFEIR, M.; SOUZA, E. M.; SIMOES-ARAUJO, J.L.; BALDANI, J.I. Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8. PLoS One, v. 13, p. e0207863-19, 2018.

 

4. MICHEL, DANIELE C.; PASSOS, SAMUEL R.; SIMOES-ARAUJO, JEAN L.; et al. Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil. Archives of Microbiology, p- 01, 2017.

 

5. DE MATOS, FEITOSA, G.; ZILLI, J.E.; SIMOES-ARAUJO, J.L. et al., Bradyrhizobium sacchari sp. nov., a legume nodulating bacterium isolated from sugarcane roots. Archives of Microbiology, v.1, p.1 – 8, 2017.

 

6. DA SILVA, P.R.A.; SIMOES-ARAUJO, J.L.; et al. Draft genome sequence of Paraburkholderia tropica Ppe8 strain, a sugarcane endophytic diazotrophic bacterium. Brazilian Journal of Microbiology, v.322, p.1 – 2, 2017.

 

7. LEITE, J.; PASSOS, S. R.; SIMOES-ARAUJO, J.L.; et al. Genomic identification and characterization of the elite strains Bradyrhizobium yuanmingense BR 3267 and Bradyrhizobium pachyrhizi BR 3262 recommended for cowpea inoculation in Brazil. Brazilian Journal of Microbiology, p.1 – 2017.

 

8. OSEI, O.; SIMOES-ARAUJO, J.L.; PCR assay for direct specific detection of Bradyrhizobium elite strain BR 3262 in root nodule extracts of soil-grown cowpea. Plant and Soil, v.2017, p.1 – 14, 2017.

 

9. POLESE, V.; SOARES, C.; DA SILVA, P.R.A.; SIMOES-ARAUJO, J.L.; et al. Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, v.1, p.1 – 14, 2017.

 

10. MELO, I.S.; PARMA, M.M.; SIMOES-ARAUJO, J.L.; et al. Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.. International Journal of Systematic and Evolutionary Microbiology (Print). 2016.

 

11. SIMOES-ARAUJO, J.L.; et al. Draft genome sequence of Bradyrhizobium yuanmingense strain BR 3267, an elite strain recommended for cowpea inoculation in Brazil. Brazilian Journal of Microbiology. v.27, p.02 – 03, 2016.

 

12. RODRIGUES, E.P.; SOARES, C.P.; GALVÃO, P.G.; IMADA, E.; SIMOES-ARAUJO, J.L.; Identification of Genes Involved in Indole-3-Acetic Acid Biosynthesis by Gluconacetobacter diazotrophicus PAL5 Strain Using Transposon Mutagenesis. Frontiers in Microbiology (Online). v.7, p.1572 – 2016.

 

13. ZILLI, J.E; MALUK, M.; REIS, V.M.; SIMOES-ARAUJO, J.L.; et al. Rhizobium altiplani sp. nov. isolated from effective nodules on Mimosa pudica growing in untypically alkaline soil in Central Brazil. International Journal of Systematic and Evolutionary Microbiology (Print). v.2016, p.2016 – 2016.

 

14. DA SILVA, P.R.A.; Vidal, M.S.; DE PAULA S.C.; POLESE, V.; SIMOES-ARAUJO, J.L.; BALDANI, José Ivo. Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice. Antonie van Leeuwenhoek (Gedrukt). v.1, p.1 – 10, 2016.

 

15. PESSOA, D.D.V.; Vidal, M.S.; BALDANI, J.I.; SIMOES-ARAUJO, J.L. Validation of reference genes for RT-qPCR analysis in Herbaspirillum seropedicae. Journal of Microbiological Methods. v.127, p.193 – 196, 2016.

 

16. SILVA, H.A.P.; NARDELI S.M.; ALVES-FERREIRA, M.; SIMOES-ARAUJO, J.L. Evaluation of Reference Genes for RT-qPCR Normalization in Cowpea under Drought Stress during Biological Nitrogen Fixation. Crop Science. v.0, p.1 – 13, 2015.

Contact information

201 Life Science Center
1201 Rollins Road
University of Missouri
Columbia, MO 65211
Email: kumarri@missouri.edu
Lab Phone No.: (573) 884 4799

EDUCATION

Ph.D. Applied Life Science

September 2011-February 2017

Gyeongsang National University, South Korea

Advisor: Prof. Jae-Yean Kim

Dissertation: “Characterization of receptor like-kinases associated with plasmodesmata in Arabidopsis thaliana”.

Masters of Science Biotechnology

August 2005-November 2007

HNB Garhwal University, India

Advisor: Dr. Alok K. Sinha (NIPGR, New Delhi, India)

Dissertation: “Cloning of MAP kinase kinase from Oryza sativa and Agrobacterium-mediated transformation”

Bachelor of Science (Botany, Zoology, and Chemistry)

June 2002-April 2005

Himachal Pradesh University India

 

RESEARCH EXPERIENCE

• May 2018-present

Postdoctoral Fellow, Stacey’s Lab, University of Missouri, Columbia, USA

• March 2017-May 2018 Postdoctoral Researcher

Cell Communication Lab, Gyeongsang National University, South Korea.

• September 2011-February 2017

Graduate Researcher, Cell Communication Lab, Gyeongsang National University, South Korea.

• February 2008-August 2009

Technical assistant, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.

• September 2009-July 2011

Senior research fellow, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.

PUBLICATIONS

1. Wu, Shu-Wei, Ritesh Kumar et al. 2018. Callose balancing at plasmodesmata. Journal of experimental botany. (Accepted)
2. Ritesh Kumar et al. 2018. OsCBSCBSPB4 is a two cystathionine-β-synthase domain-containing protein from rice that functions in abiotic stress tolerance. Current Genomics 19(1): 50-59.
3. Dhinesh Kumar, Ritesh Kumar et al. 2017. A leucine-rich repeat receptor-like kinase RECEPTOR DEAD KINASE1 (RDK1) mediates the interaction of type 2C protein phosphatase ABI1 with plasma membrane and positively regulates ABA responses in Arabidopsis thaliana. Molecular Plant 10(2): 223-243. (Equal first author).
4. Rohit Joshi, Khirod Kumar Sahoo, Amit Kumar Tripathi, Ritesh Kumar et al. 2017. Knockdown of an inflorescence meristem-specific cytokinin oxidase-OsCKX2 in rice reduces yield penalty under salinity stress condition. Plant, Cell & Environment doi: 10.1111/pce.12947.
5. Ritesh Kumar et al. 2016. A Strategy to validate the role of callose-mediated plasmodesmal gating in the tropic response. Journal of Visualized Experiments (110), e53513, doi:10.3791/53513.
6. Anil K Singh, Ritesh Kumar et al. 2015. Genome-wide investigation and expression analysis of Sodium/Calcium exchanger gene family in rice and Arabidopsis. Rice 8:21. doi:10.1186/s12284-015-0054-5. (Equal first author).
7. Ritesh Kumar et al. 2015. Players at plasmodesmal nano-channels. Journal of Plant Biology 58(2):75-86.
8. Xiao Han, Tae Kyung Hyun, M Zhang, Ritesh Kumar et al. 2014. Auxin-callose-mediated plasmodesmal gating is essential for tropic auxin gradient formation and signaling. Developmental Cell 28(2):132-146.
9. Dhinesh Kumar, Ritesh Kumar et al. 2014. Cell-to-cell movement of viruses via plasmodesmata. Journal of Plant Research 128(1). doi:10.1007/s10265-014-0683-6. (Equal first author).
10. Tae Kyung Hyun, Sarah Lee, Yeonggil Rim, Ritesh Kumar et al. 2014. De-novo RNA sequencing and metabolite profiling to identify genes involved in anthocyanin biosynthesis in Korean Black Raspberry (Rubus coreanus Miquel). PLoS ONE 9(2): e88292. doi:10.1371/journal.pone.0088292.
11. Tae Kyung Hyun, Sarah Lee, Dhinesh Kumar, Yeongil Rim, Ritesh Kumar et al. 2014. RNA-seq analysis of Rubus idaeus cv. Nova: transcriptome sequencing and de novo assembly for subsequent functional genomics approaches. Plant Cell Reports 33(10):1617-28.
12. Dhinesh Kumar, Ritesh Kumar et al. 2013. Plasmodesmata and phloem-based trafficking of macromolecules. Symplasmic Transport in Vascular Plants, Edited by Katarzyna Sokołowska and Paweł Sowiński,: chapter Plasmodesmata and phloem-based trafficking of macromolecules: Springer New York ISBN: 978-1-4614-7765-5, 183-216.
13. Xiao Han, Cong Chen, Tae Kyung Hyun, Ritesh Kumar et al. 2012. Metabolic module mining based on independent component analysis in Arabidopsis thaliana. Molecules & Cells 34(3):295-304.
14. Tae Kyung Hyun, Yeonggil Rim, Hui-Jeong Jang, Cheol Hong Kim, Jongsun Park, Ritesh Kumar et al. 2012. De novo transcriptome sequencing of Momordica cochinchinensis to identify genes involved in the carotenoid biosynthesis. Plant Molecular Biology 79(4-5):413-27.
15. Ritesh Kumar et al. 2012. Functional screening of cDNA library from a salt tolerant rice genotype Pokkali identifies mannose-1-phosphate guanyl transferase gene (OsMPG1) as a key member of salinity stress response. Plant Molecular Biology 79(6): 555-68.
16. Anil K Singh, Ritesh Kumar et al. 2012. Overexpression of rice CBS domain containing protein improves salinity, oxidative, and heavy metal tolerance in transgenic tobacco. Molecular biotechnology 52 (3), 205-216.

 

 

 

 

Contact information:

Adama R Tukuli

201 Life Science Center
1201 Rollins Road
University of Missouri
Columbia, MO 65211
Email: artkv6@mail.missouri.edu
Lab Phone No.: (573) 884 4799

 

Education:

2004-2007 –BS – Plant Sciences, Jimma University, Ethiopia

2017–Present Ph. D student in Plant breeding, genetics and genomics, University of Missouri.

Work Experience

2013-2016 Research lab technician at University of Missouri Columbia,

2011-2013 Research Lab Technician at Monsanto in Soybean breeding, St. Louis MO, USA.

2008-2010 Research lab technician at Honnover University, Germany

2007-2008 Graduate assistant lecturer at Arba Minch University, Ethiopia

 

 

 

 

 

CONTACT INFORMATION:

MIKI HODEL

Email: mfhxv8@mail.missouri.edu 

Education

Bachelor of Science in Biology:  Graduation Date: May 2020, University of Missouri-Columbia  

Relevant Experience

Legume-Microbe Interactions Laboratory of Dr. Gary Stacey and Dr. Bing Stacey

October 2017- May 2018

Undergraduate Research Assistant University of Missouri