Hartanto, Christopher F.

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Contact information

Tadra Sfeir, Michelle

202 Life Science Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: tadrasfeirm@missouri.edu

Lab : (573) 884 4799

Education:

2002-2005 – B.S – Biological Sciences

Federal University of Parana, Brazil

2006-2008 – M.SC – Biochemistry and Molecular Biology

Federal University of Parana, Brazil

March 2008- November 2011 – Ph.D  – Biochemistry and Molecular Biology

Federal University of Parana, Brazil

Thesis topic: Functional characterization of Herbaspirillum seropedicae genes regulated by flavonoid naringenin

Research focus:

Plant-bacteria nitrogen fixation interaction.

Professional Experience:

December 2011 – May 2017 Postdoctoral Research

Biochemistry and Molecular Biology

Federal University of Parana, Brazil

Current research focus:

Associations between soybean with nitrogen fixing bacteria.

Publications:

1. PIE, MARCIO R.; STR?HER, PATR?CIA R.; AGOSTINIS, ANDR? O.; BELMONTE-LOPES, RICARDO; Tadra-Sfeir, Michelle Z.; OSTRENSKY, ANTONIO Development of a real-time PCR assay for the detection of the golden mussel (Limnoperna fortunei, Mytilidae) in environmental samples. ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS. , v.89, p.1041 – 1045, 2017.
2. LEAO, ANIELE C. RIBAS; WEISS, VINICIUS ALMIR; VICENTE, VANIA APARECIDA; COSTA, FLAVIA; BOMBASSARO, AMANDA; RAITTZ, ROBERTO TADEU; Steffens, Maria Berenice R.; PEDROSA, FABIO OLIVEIRA; GOMES, RENATA R.; BAURA, VALTER; Faoro, Helisson; SFEIR, MICHELLE ZIBETTI TADRA; Balsanelli, Eduardo; MORENO, LEANDRO F.; NAJAFZADEH, M. JAVAD; DE HOOG, SYBREN; SOUZA, EMANUEL MALTEMPI Genome Sequence of Type Strain Fonsecaea multimorphosa CBS 980.96 T , a Causal Agent of Feline Cerebral Phaeohyphomycosis. Genome Announcements. , v.5, p.e01666-16 – , 2017.
3. DE SOUZA GODOI, POLIANA ALVES; PIECHNIK, CLAUDIO ADRIANO; DE OLIVEIRA, ANA CAROLINE; SFEIR, MICHELLE ZIBETTI; DE SOUZA, EMANUEL MALTEMPI; ROGEZ, HERVÉ; THOMAZ SOCCOL, VANETE qPCR for the detection of foodborne Trypanosoma cruzi. PARASITOLOGY INTERNATIONAL. , v.16, p.1383 – , 2017.
4. ALVES, LUIS P. S.; TEIXEIRA, CÍCERO S.; TIRAPELLE, EVANDRO F.; DONATTI, LUCÉLIA; TADRA-SFEIR, M. Z.; STEFFENS, MARIA B. R.; de Souza, Emanuel M.; DE OLIVEIRA PEDROSA, FABIO; Chubatsu, Leda S.; MÜLLER-SANTOS Marcelo Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation. Frontiers in Microbiology (Online). , v.7, p.739 – , 2016.
5. BOMBASSARO, AMANDA; DE HOOG, SYBREN; Weiss, Vinicius A.; Souza, Emanuel M.; LEÃO, ANIELE C. R.; COSTA, FLÁVIA F.; BAURA, VALTER; TADRA-SFEIR, MICHELE Z.; Balsanelli, Eduardo; MORENO, LEANDRO F.; Raittz, Roberto T.; Steffens, Maria Berenice R.; Pedrosa, Fabio O.; SUN, JIUFENG; XI, LIYAN; BOCCA, ANAMÉLIA L.; FELIPE, MARIA S.; TEIXEIRA, MARCUS; SANTOS, GERMANA D.; TELLES FILHO, FLÁVIO Q.; AZEVEDO, CONCEIÇÃO M. P. S.; GOMES, RENATA R.; VICENTE, VÂNIA A. Draft Genome Sequence of Fonsecaea monophora Strain CBS 269.37, an Agent of Human Chromoblastomycosis. Genome Announcements. , v.4, p.e00731-16 – , 2016.
6. COSTA, FLÁVIA F.; DE HOOG, SYBREN; Raittz, Roberto T.; Weiss, Vinicius A.; LEÃO, ANIELE C. R.; BOMBASSARO, AMANDA; SUN, JIUFENG; MORENO, LEANDRO F.; Souza, Emanuel M.; Pedrosa, Fabio O.; Steffens, Maria Berenice R.; BAURA, VALTER; TADRA-SFEIR, MICHELE Z.; Balsanelli, Eduardo; NAJAFZADEH, M. JAVAD; GOMES, RENATA R.; FELIPE, MARIA S.; TEIXEIRA, MARCUS; SANTOS, GERMANA D.; XI, LIYAN; ALVES DE CASTRO, MAURO ANTÔNIO; VICENTE, VÂNIA A. Draft Genome Sequence of Fonsecaea nubica Strain CBS 269.64, Causative Agent of Human Chromoblastomycosis. Genome Announcements. , v.4, p.e00735-16 – , 2016.
7. MESA, D.; LOURENCO, M.; BUENO, A.; TADRA-SFEIR, MICHELLE Z; SANTIN, E.
   Influence of covering reused broiler litter with plastic sheet on litter characteristics and bacteriology and the subsequent immunity and microbiology of chickens housed on the litter. Revista Brasileira de Ciencia Avicola / Brazilian Journal of Poultry Science. , v.10, p.1-19 – , 2016.
8. SPERB, EDILENA REIS; TADRA-SFEIR, MICHELLE ZIBETTI; SPEROTTO, RAUL ANTÔNIO; FERNANDES, GABRIELA DE CARVALHO; PEDROSA, FÁBIO DE OLIVEIRA; DE SOUZA, EMANUEL MALTEMPI; PASSAGLIA, LUCIANE MARIA PEREIRA Iron deficiency resistance mechanisms enlightened by gene expression analysis in Paenibacillus riograndensis SBR5. Research in Microbiology (Paris). , v.167, p.501 – 509, 2016.
9. BONATO, PALOMA; BATISTA, MARCELO B.; CAMILIOS-NETO, DOUMIT; PANKIEVICZ, VÂNIA C. S.; TADRA-SFEIR, M. Z.; MONTEIRO, Rose Adele; Pedrosa, Fabio O.; Souza, Emanuel M.; Chubatsu, Leda S.; WASSEM, Roseli; RIGO, Liu Un RNA-seq analyses reveal insights into the function of respiratory nitrate reductase of the diazotroph Herbaspirillum seropedicae. Environmental Microbiology (Print). , v.18, p.000 – , 2016.
10. PANKIEVICZ, V. C. S.; CAMILIOS-NETO, D.; BONATO, P.; BALSANELLI, E.; TADRA-SFEIR, M. Z.; FAORO, H.; CHUBATSU, L. S.; DONATTI, L.; WAJNBERG, G.; PASSETTI, F.; MONTEIRO, R. A.; PEDROSA, F. O.; SOUZA, E. M. RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots. Plant Molecular Biology. , v.1, p.1 – 15, 2016.
11. Faoro, Helisson; RENE MENEGAZZO, RODRIGO; BATTISTONI, FEDERICO; GYANESHWAR, PRASAD; DO AMARAL, FERNANDA P.; TAULÉ, CECILIA; RAUSCH, SYDNEE; GONÇALVES GALVÃO, PATRICIA; DE LOS SANTOS, CECILIA; MITRA, SHUBHAJIT; HEIJO, GABRIELA; SHEU, SHIH-YI; CHEN, WEN-MING; MAREQUE, CINTIA; TADRA-SFEIR, M. Z.; IVO BALDANI, J.; MALUK, MARTA; PAULA GUIMARÃES, ANA; STACEY, GARY; SOUZA, E. M.; Pedrosa, Fabio O.; Cruz, Leonardo Magalhães; JAMES, EUAN K. The oil-contaminated soil diazotroph Azoarcus olearius DQS-4 T is genetically and phenotypically similar to the model grass endophyte Azoarcus sp. BH72. Environmental Microbiology Reports. , v.on lin, p.1 – 16, 2016.
12. GUIZELINI, DIEVAL; SAIZAKI, PAULA M.; COIMBRA, NILSON A. R.; Weiss, Vinicius A.; Faoro, Helisson; SFEIR, MICHELLE Z. T.; Baura, Valter A.; Monteiro, Rose A.; Chubatsu, Leda S.; Souza, Emanuel M.; Cruz, Leonardo M.; Pedrosa, Fabio O.; Raittz, Roberto T.; MARCHAUKOSKI, JERONIZA N.; STEFFENS, MARIA B. R. Complete Genome Sequence of Herbaspirillum hiltneri N3 (DSM 17495), Isolated from Surface-Sterilized Wheat Roots. Genome Announcements. , v.3, p.e01288-15 – , 2015.
13. DE SOUZA, ROCHELI; SANT’ANNA, FERNANDO HAYASHI; AMBROSINI, ADRIANA; TADRA-SFEIR, MICHELE; Faoro, Helisson; PEDROSA, FABIO OLIVEIRA; SOUZA, EMANUEL MALTEMPI; PASSAGLIA, LUCIANE M. P. Genome of Pseudomonas sp. FeS53a, a Putative Plant Growth-Promoting Bacterium Associated with Rice Grown in Iron-Stressed Soils. Genome Announcements. , v.3, p.e00248-15 – , 2015.
14. DE SOUZA, ROCHELI; SANT’ANNA, FERNANDO HAYASHI; AMBROSINI, ADRIANA; TADRA-SFEIR, MICHELE; Faoro, Helisson; PEDROSA, FABIO OLIVEIRA; SOUZA, EMANUEL MALTEMPI; PASSAGLIA, LUCIANE M. P. Genome of Rhizobium sp. UR51a, Isolated from Rice Cropped in Southern Brazilian Fields. Genome Announcements. , v.3, p.e00249-15 – , 2015.
15. AMBROSINI, ADRIANA; SANT’ANNA, FERNANDO HAYASHI; DE SOUZA, ROCHELI; TADRA-SFEIR, MICHELE; Faoro, Helisson; ALVARENGA, SAMUEL M.; PEDROSA, FABIO OLIVEIRA; SOUZA, EMANUEL MALTEMPI; PASSAGLIA, LUCIANE M. P. Genome Sequence of Bacillus mycoides B38V, a Growth-Promoting Bacterium of Sunflower. Genome Announcements. , v.3, p.e00245-15 – , 2015.
16. Tadra-Sfeir, Michelle Z.; Faoro, Helisson; CAMILIOS-NETO, DOUMIT; BRUSAMARELLO-SANTOS, LIZIANE; Balsanelli, Eduardo; WEISS, VINICIUS; Baura, Valter A.; Wassem, Roseli; Cruz, Leonardo M.; DE OLIVEIRA PEDROSA, FÁBIO; Souza, Emanuel M.; Monteiro, Rose A.
    Genome wide transcriptional profiling of Herbaspirillum seropedicae SmR1 grown in the presence of naringenin. Frontiers in Microbiology (Online). , v.6, p.www.frontiersin – , 2015.
17. BALSANELLI, Eduardo; TADRA-SFEIR, M. Z.; FAORO, Helisson; PANKIEVICZ, VÂNIA C.S.; de Baura, Valter A.; Pedrosa, Fábio O.; de Souza, Emanuel M.; DIXON, RAY; Monteiro, Rose A.
    Molecular adaptations of Herbaspirillum seropedicae during colonization of the maize rhizosphere. Environmental Microbiology (Print). , v.1, p.n/a – n/a, 2015.
18. PEREIRA, JAMILE QUEIROZ; AMBROSINI, ADRIANA; SANT’ANNA, FERNANDO HAYASHI; TADRA-SFEIR, MICHELE; Faoro, Helisson; PEDROSA, FÁBIO OLIVEIRA; SOUZA, EMANUEL MALTEMPI; BRANDELLI, ADRIANO; PASSAGLIA, LUCIANE M. P. Whole-Genome Shotgun Sequence of the Keratinolytic Bacterium Lysobacter sp. A03, Isolated from the Antarctic Environment. Genome Announcements. , v.3, p.e00246-15 – , 2015.
19. CAMILIOS-NETO, DOUMIT; BONATO, PALOMA; Wassem, Roseli; TADRA-SFEIR, MICHELLE Z; BRUSAMARELLO-SANTOS, LIZIANE CC; VALDAMERI, GLAUCIO; DONATTI, LUCÉLIA; Faoro, Helisson; WEISS, VINICIUS A; CHUBATSU, LEDA S; PEDROSA, FÁBIO O; SOUZA, EMANUEL M Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes. BMC Genomics. , v.15, p.378 – , 2014.
20. SMIDERLE, FHERNANDA R.; ALQUINI, GIOVANA; Tadra-Sfeir, Michelle Z.; IACOMINI, MARCELLO; WICHERS, HARRY J.; VAN GRIENSVEN, LEO J.L.D. Agaricus bisporus and Agaricus brasiliensis (1→6)-β-d-glucans show immunostimulatory activity on human THP-1 derived macrophages. Carbohydrate Polymers. , v.94, p.91 – 99, 2013.
21. DE SOUZA, V.; PIRO, V. C.; Faoro, H.; Tadra-Sfeir, M. Z.; CHICORA, V. K.; GUIZELINI, D.; WEISS, V.; VIALLE, R. A.; Monteiro, R. A.; Steffens, M. B. R.; MARCHAUKOSKI, J. N.; Pedrosa, F. O.; Cruz, L. M.; Chubatsu, L. S.; Raittz, R. T. Draft Genome Sequence of Herbaspirillum huttiense subsp. putei IAM 15032, a Strain Isolated from Well Water. Genome Announcements. , v.1, p.e00252-12 – e00252-12, 2013.
22. TIRAPELLE, EVANDRO F.; Müller-Santos, Marcelo; TADRA-SFEIR, M. Z.; KADOWAKI, MARCO A. S.; STEFFENS, MARIA B. R.; Monteiro, Rose A.; Souza, Emanuel M.; SOUZA, E. M.; Pedrosa, Fabio O.; Chubatsu, Leda S. Identification of Proteins Associated with Polyhydroxybutyrate Granules from Herbaspirillum seropedicae SmR1 – Old Partners, New Players. Plos One. , v.8, p.e75066 – , 2013.
23. CORDEIRO, FABIO APARECIDO; TADRA-SFEIR, MICHELLE ZIBETTI; HUERGO, LUCIANO FERNANDES; DE OLIVEIRA PEDROSA, FÁBIO; Monteiro, Rose Adele; DE SOUZA, EMANUEL MALTEMPI. Proteomic Analysis of Herbaspirillum seropedicae Cultivated in the Presence of Sugar Cane Extract. Journal of Proteome Research (Online). , v.12, p.1142 – 1150, 2013.
24. BATISTA, MARCELO B.; SFEIR, MICHELLE Z. T.; Faoro, Helisson; Wassem, Roseli; Steffens, Maria B. R.; Pedrosa, Fábio O.; Souza, Emanuel M.; DIXON, RAY; Monteiro, Rose A. The Herbaspirillum seropedicae SmR1 Fnr orthologs controls the cytochrome composition of the electron transport chain. Scientific Reports. , v.3, p.2544 – , 2013.
25. Camboim, Expedito K. A.; Tadra-Sfeir, Michelle Z.; de Souza, Emanuel M.; Pedrosa, Fabio de O.; Andrade, Paulo P.; McSweeney, Chris S.; Riet-Correa, Franklin; Melo, Marcia A. Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil. The Scientific World Journal. , v.2012, p.1 – 5, 2012.
26. Weiss, V. A.; Faoro, H.; Tadra-Sfeir, M. Z.; Raittz, R. T.; de Souza, E. M.; Monteiro, R. A.; Cardoso, R. L. A.; Wassem, R.; Chubatsu, L. S.; Huergo, L. F.; Muller-Santos, M.; Steffens, M. B. R.; Rigo, L. U.; Pedrosa, F. d. O.; Cruz, L. M. Draft Genome Sequence of Herbaspirillum lusitanum P6-12, an Endophyte Isolated from Root Nodules of Phaseolus vulgaris. Journal of Bacteriology (Print). , v.194, p.4136 – 4137, 2012.
27. Monteiro, Rose A.; Balsanelli, Eduardo; Tuleski, Thalita; Faoro, Helison; Cruz, Leonardo M.; Wassem, Roseli; Baura, Valter A.; Tadra-Sfeir, Michelle Z.; Weiss, Vinícius; DaRocha, Wanderson D.; Muller-Santos, Marcelo; Chubatsu, Leda S.; Huergo, Luciano F.; Pedrosa, Fábio O.; Souza, Emanuel M. Genomic comparison of the endophyte Herbaspirillum seropedicae SmR1 and the phytopathogen Herbaspirillum rubrisubalbicans M1 by Suppressive Subtractive Hybridization and partial genome sequencing. FEMS Microbiology, Ecology (Print). , v.xx, p.n/a – n/a, 2012.
28. Monteiro, Rose Adele; Balsanelli, Eduardo; Wassem, Roseli; Marin, Anelis M.; Brusamarello-Santos, Liziane C. C.; Schmidt, Maria Augusta; Tadra-Sfeir, Michelle Z.; Pankievicz, V̢nia C. S.; Cruz, Leonardo M.; Chubatsu, Leda S.; Pedrosa, Fabio O.; Souza, Emanuel M. Herbaspirillum-plant interactions: microscopical, histological and molecular aspects. Plant and Soil (Print). , v.x, p.x Р, 2012.
29. Camboim, Expedito K. A.; Almeida, Arthur P.; Tadra-Sfeir, Michelle Z.; Junior, Felício G.; Andrade, Paulo P.; McSweeney, Chris S.; Melo, Marcia A.; Riet-Correa, Franklin Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil. The Scientific World Journal. , v.2012, p.1 – 6, 2012.
30. Pedrosa, Fábio O.; Monteiro, Rose Adele; Wassem, Roseli; Cruz, Leonardo M.; Ayub, Ricardo A.; Colauto, Nelson B.; Fernandez, Maria Aparecida; Fungaro, Maria Helena P.; Grisard, Edmundo C.; Hungria, Mariangela; Madeira, Humberto M. F.; Nodari, Rubens O.; Osaku, Clarice A.; Petzl-Erler, Maria Luiza; Terenzi, Hernán; Vieira, Luiz G. E.; Steffens, Maria Berenice R.; Weiss, Vinicius A.; Pereira, Luiz F. P.; Almeida, Marina I. M.; Alves, Lysangela R.; Marin, Anelis; Araujo, Luiza Maria; Balsanelli, Eduardo; Baura, Valter A.; Chubatsu, Leda S.; Faoro, Helisson; Favetti, Augusto; Friedermann, Geraldo; Glienke, Chirlei; Karp, Susan; Kava-Cordeiro, Vanessa; Raittz, Roberto T.; Ramos, Humberto J. O.; Ribeiro, Enilze Maria S. F.; Rigo, Liu Un; Rocha, Saul N.; Schwab, Stefan; Silva, Anilda G.; Souza, Eliel M.; Tadra-Sfeir, M. Z. Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses. PLOS Genetics (Online). , v.7, p.e1002064 – , 2011.
31. TADRA-SFEIR, M. Z.; Souza, E. M.; Faoro, H.; Muller-Santos, M.; Baura, V. A.; Tuleski, T. R.; Rigo, L. U.; Yates, M. G.; Wassem, R.; Pedrosa, F. O.; Monteiro, R. A. Naringenin Regulates Expression of Genes Involved in Cell Wall Synthesis in Herbaspirillum seropedicae. Applied and Environmental Microbiology (Print). , v.77, p.2180 – 2183, 2011.

CONTACT INFORMATION:

Caroline Kukolj

Research Scholar – Ph.D. Student

201 Life Science Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: kukoljc@missouri.edu

Lab phone: (573) 884 4799

EDUCATION

2005-2009 BS – Biological Sciences- Federal University of Paraná-UFPR- Curitiba-Brazil.

2010-2012 MS – Genetics – Federal University of Paraná-UFPR- Curitiba-Brazil.

2014-present – PhD Student of Biochemistry Sciences – Federal University of Paraná-Curitiba-Brazil. Thesis topic: Differential proteomics analysis  of Azospirillum brasilense and its mutants glnB, glnZ, glnB:glnZ and ntrC after growth under high and low ammonium conditions

CURRENT PROJECT

Differential metabolomic analysis of Azospirillum brasilense and its mutants glnB, glnZ, glnB:glnZ and ntrC after growth under high and low ammonium conditions

PUBLICATION

FIORINI, A. ; ROSADO, F. R. ; BETTEGA, E. M. ; SIBIN, K. C.; KUKOLJ, C.; BONFIM-MENDONÇA, PATRICIA DE SOUZA ; SHINOBU-MESQUITA, Cristiane Suemi ; GHIRALDI-LOPES, L. D. ; Campanerut-Sá, P.A.Z. ; CAPOCI, I. R. G. ; GODOY, J. S. R. ; FERREIRA, I. C. P. ; SVIDZINSKI, TEREZINHA I. E. . Candida albicans PROTEIN PROFILE CHANGES IN RESPONSE TO THE BUTANOLIC EXTRACT OF Sapindus saponaria L.. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 1, 2016.

COSTA, G. G. ; GOMIG, T. H. B. ; KAVISKI, R. ; SOUSA, K. S. ; KUKOLJ, C.; LIMA, R. S. ; URBAN, C. A. ; CAVALLI, I. J. ; RIBEIRO, E. M. S. F. . Comparative Proteomics of Tumor and Paired Normal Breast Tissue Highlights Potential Biomarkers in Breast Cancer. Cancer Genomics & Proteomics , v. 12, p. 251-262, 2015.

CONTACT INFORMATION:

STERLING EVANS
Email: scecpb@mail.missouri.edu
Cell: (816) 442-1920

Education

Bachelor of Science in Plant Science Graduation Date: May 2020, University of Missouri-Columbia (GPA: 4.00)
• Minors: Biology, Sustainable Agriculture
• Bright Flight Scholar: Top 3% of Missouri High School Seniors
• Chancellor’s Award winner: University of Missouri-Columbia, minimum ACT score of 31

Lathrop High School: Honors Diploma Graduation Date: May 2016
• Unweighted GPA: 3.98/4.00
• Class Salutatorian

Relevant Experience

Legume-Microbe Interactions Laboratory of Dr. Gary Stacey October 2016-Present
Undergraduate Research Assistant University of Missouri

• Gained laboratory techniques: making media, growing plants, specifically Setaria viridis and soybean (Glycine max (L.) Merr.) in greenhouses and growth chambers, rhizobacteria (wildtype and mutant strains) culture maintenance, bacteria inoculation of plant seedlings, measuring root parameters using the WinRHIZO software program, DNA extraction
• Developed skills using laboratory instruments: Centrifuge, Autoclave, Gel Electrophoresis, Vibratome 3000 Plus for sectioning samples, Shaking incubator for bacteria maintenance, Genesys 10s Spectrophotometer for measuring the optical density of bacteria
• Logged daily methods, results and procedures
• Developed Seteria Viridis mutant screening
• Analyzed mass spectra data like multivariate statistical and hierarchical clustering analyses from metabolomics
• Carried out DNA/RNA extractions with Arabidopsis

Drought Tolerance Laboratory of Dr. Robert VanBuren May-August 2017
Summer Research Assistant Michigan State University
• Gene Cloning, E. Coli transformation, Floral Dipping, PCR
• Carried out RNA sequencing data analysis for comparative genomics study
• Developed a protocol for a tractable E. coli desiccation assay
• Ethyl methanesulfonate (EMS) mutagenesis of Eragrostis tef
• Became comfortable running programs on Unix command line

Presentations

Back to the Columns Poster Presentation: University of Missouri September 2017
Using resurrection species to bring new life to drought tolerance research
• Back to the Columns is a presentation specific to undergraduates who carried out summer research outside of the University of Missouri Campus.
• I presented the research that I carried out at Michigan State University, where I spent my summer.

Mid-Sure Poster Presentation: Michigan State University July 2017
Using resurrection species to bring new life to drought tolerance research
• Mid-Sure is open to all summer undergraduate research, within any field, carried out on the MSU campus.
• I presented the date gathered from my 10-week Research Experience for Undergraduates (REU) in the Dr. Robert VanBuren Lab.

Life Science Week Poster Presentation: University of Missouri April 2017
Phenotypic screening of Setaria viridis associated with plant growth promoting bacteria
• Life Science Week gathers researchers ranging from undergraduates to post-doctoral researchers who work within the life science at the University of Missouri
• I presented relevant preliminary data gathered from my work in the Dr. Gary Stacey Lab

Honors

• Undergraduate Research Excellence awarded during the University of Missouri’s Life Science Week 2017.
• Accepted in to the Freshman Research in Plant Science program (FRIPS). The FRIPS program is a selective program that only accepted 7 of the top freshman researchers in 2016.
• Featured in Decoding Science. A publication through the Bond Life Science Center at the University of Missouri.

CONTACT INFORMATION:

Luis Paulo Silveira Alves

Research Scholar – Ph.D. Student

201 Life Science Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: alvesl@missouri.edu

Lab phone: (573) 884 4799

EDUCATION:

2007-2011 –BS – Biological Sciences- Federal University of Paraná-UFPR- Curitiba-Brazil.

2012-2014 – MS- Biochemistry Sciences- Federal University of Paraná-Curitiba-Brazil.

2014-present –PhD Student of Biochemistry Sciences – Federal University of Paraná-Curitiba-Brazil. Thesis topic: Investigation of the Role of Phasin Proteins in Polyhydroxyalkanoate Biosynthesis and in the Anti-Stress Response in Bacteria Advisor: Dr. Marcelo Müller dos Santos.

March, 2017-present – Research Scholar – Division of Plant Sciences, University of Missouri.

CURRENT PROJECT:

Plant-bacteria interaction with the model plants lant-bacteria interaction with the model plants Brachypodium distachyum and Setaria viridis. 

Advisor: Dr. Gary Stacey

PUBLICATIONS: 

ALVES, L.P.S. ; ALMEIDA, A.T. ; CRUZ, L.M. ; PEDROSA, F.O. ; DE SOUZA, E.M. ; CHUBATSU, L.S.; MÜLLER-SANTOS, M. ; VALDAMERI, G. A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry. Brazilian journal of medical and biological research, v. 50, p. e5492, 2017.

ALVES, LUIS P. S.; TEIXEIRA, CÍCERO S.; TIRAPELLE, EVANDRO F.; DONATTI, LUCÉLIA; Tadra-Sfeir, Michelle Z.; STEFFENS, MARIA B. R.; de Souza, Emanuel M.; DE OLIVEIRA PEDROSA, FABIO; Chubatsu, Leda S.; Müller-Santos, Marcelo. Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation. Frontiers in Microbiology (Online), v. 7, p. 739, 2016.

CONTACT INFORMATION

Division of Plant Sciences

201 Christopher S. Bond Life Sciences Center

1201 E. Rollins Street

University of Missouri-Columbia

Columbia MO 65211

Email: ptdc4c@mail.missouri.edu

Lab phone: (573) 884 4799

EDUCATION

2016 Ph.D.     University of Missouri

2009 M.S.       Vietnam National University of Agriculture

2003 B.S.        Vietnam National University of Agriculture

PROFESSIONAL EXPERIENCES

November 2003 –July 2011:

Research Assistant: Institute of Biotechnology, Vietnam Academy of Science and Technology

August 2011 – June 2016:

Ph.D. Student: Division of Plant Science, University of Missouri

CURRENT RESEARCH FOCUS

Transposon mutants and CRISPR/Cas9 targeted mutagenesis technologies in soybean.

PUBLICATIONS

Stacey MG. Cahoon RE, Nguyen HT, Nguyen CT, Cui Y, Sato S, Phoka N, Clark KM, Liang Y, Forrester J, Batek J, Do PT, Sleper DA, Clemente TE, Cahoon EB, Stacey G (2016) Identification of Homogentisate Dioxygenase as a Target for Vitamin E Biofortification in Oilseeds. Plant Physiol. 172(3):1506-1518

Do P, Lee HY, Mookkan M, Folk WR, Zhang Z (2016) Rapid and efficient Agrobacterium-mediated transformation of sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker. Plant Cell Rep. 35(10):2065-76

Do P, De Tar JR, Lee H, Folta M, Zhang Z (2016) Expression of ZmGA20ox cDNA alters plant morphology and increases biomass production of switchgrass (Panicum virgatum L.). Plant Biotechnol J. 14(7):1532-40

Do P, Zhang Z (2015) Sorghum transformation: Achievements, challenges, and perspectives. In: Recent advancements in gene expression and enabling technologies in crop plants. Azhakanandam K, Silverstone A, Daniell H, Davey MR, ed. Springer-Verlag, New York, p1-455.

CONTACT INFORMATION:

Trang Le Van

Visiting Scholar at the Stacey Lab

202 Life Sciences Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: trangl@missouri.edu

Lab phone No.: 573-884-4799

EDUCATION:

– 2007-2011: Bachelor in Agriculture, Mekong University, Vietnam. Major: Biotechnology. Thesis title: “Application of Marker-Assisted Selection in Fragrant Rice Breeding and Selection”.

PROFESSIONAL EXPERIENCES:

– Basic skills in soybean and rice breeding and variety selection.
– DNA extraction, PCR.
– Good skills with Minitab, Microsoft Word, Excel, Powerpoint.

EMPLOYMENT:

– September 2011 – March 2016: Researcher, Department of Plant Genetics and Breeding, the Cuu Long Delta Rice Research Institute, Cantho, Vietnam.

– March 2016 – Now: Visiting Scholar, Division of Plant Sciences, University of Missouri, Columbia, MO, USA

CURRENT PROJECT:

– A resource for functional genomics to support soybean genetics and breeding (NSF grant)

– Identification and characterization of novel plant genotypes for soybean seed trait improvement using fast neutron irradiated mutants (MSMC grant)

– Characterization of genetic lesions and yield potential of two fast neutron mutants with high seed-per-pod value traits (MSMC grant)

– Identification and characterization of novel plant genotypes for soybean seed trait improvement
using fast neutron irradiated mutants (MSMC grant)

– Network-guided modification of soybean meal composition (United Soybean Board grant)

CONTACT INFORMATION:

Mariola Klepadlo

Division of Plant Sciences,
201 C.S. Bond Life Science Center, 1201 Rollins St.
University of Missouri, Columbia, MO 65211
Lab Phone No.: (573) 884 4799

Fax : + 1 573-884-9676
E-mail: klepadlom@missouri.edu

SUMMARY:

Strong dual background in classical and molecular plant breeding. Highly skilled in leading and managing field and lab activities toward crop line development and release. Versatile professional with strong written and verbal communication and technical skills.

EDUCATION:

Ph.D. in Soybean Breeding and Genetics (2012 – 2015), University of Arkansas, Fayetteville, AR.

M.S. in Horticultural Genetics and Biotechnology (2009 – 2011), Mediterranean Agronomic Institute of Chania, Greece.

M.S. in Plant Biotechnology (2007 – 2009), University of Szczecin, Poland.

B.S. in Biotechnology (2004 – 2007), University of Szczecin, Poland.

PROFESSIONAL EXPERIENCE:

Post-doctoral Fellow (February 2016 – present); Division of Plant Sciences, C. S. Bond Life Science Center, National Center for Soybean Biotechnology, University of Missouri, Columbia, MO.

Senior Research Assistant (July 2012 – January 2016); Soybean Breeding and Genetics, Crop, Soil, and Environmental Sciences, Plant Sciences Building, University of Arkansas, Fayetteville, AR.

Research Assistant (September 2010 – June 2012); Laboratory of Molecular Biology and Biotechnology (GMO Section),  Department f Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute of Chania, Greece.

CURRENT RESEARCH:

Development and characterization of fast neutron and transposon mutants in soybean utilized as a genetic resources to the soybean community for gene discovery, genetic diversity and agronomic improvement.

Leading soybean mutant lines development at research stations in MO.

Mapping yield and seed composition genes.

CONTACT INFORMATION:

Thalita Regina Tuleski (Full CV)

Visiting Ph.D. Student at the Stacey Lab

201 Life Sciences Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: tuleskit@missouri.edu

Lab phone No.: 573-884-4799

EDUCATION:

2013-current: Ph.D. in progress in Sciences (Biochemistry). Federal University of Paraná, UFPR, Brazil.
– Advisor: Rose Adele Monteiro.
– Grantee of: CNPq

2011-2013: Master´s in Sciences (Biochemistry). Federal University of Paraná, UFPR, Brazil.
Year of degree: 2013.
– Advisor: Rose Adele Monteiro.
– Grantee of: CAPES

2007-2010: Graduation in Biological Sciences. Federal University of Paraná, UFPR, Brazil
– Advisor: Rose Adele Monteiro.
– Grantee of: CNPq

PROFESSIONAL EXPERIENCES:

2012-Current: Graduate student , Position: Scholar CAPES , Working hours (weekly): 40, Schemes of job: Full-time and exclusiveness. Federal University of Santa Catarina – UFSC.

2011: Internship , Position: Intern , Working hours (weekly): 40, Schemes of job: Full-time and exclusiveness. Dow AgroSciences – DOWAGRO

2010 & 2011: Scientific Initiation Scholarship, Position: Scholarship FAPESC, Working hours (weekly): 20, Schemes of job: Full-time and exclusiveness. Universidade do Estado de Santa Catarina – UDESC

2008-2009: Scientific Initiation Scholarship, Position: Scholarship FAPESC, Working hours (weekly): 20, Schemes of job: Full-time and exclusiveness. Universidade do Estado de Santa Catarina – UDESC

PUBLICATIONS:

Articles in Scientific Journals

1. Balsanelli, Eduardo; Tuleski, Thalita Regina ; De Baura, Valter Antonio ; Yates, Marshall Geoffrey ; Chubatsu, Leda Satie ; De Oliveira Pedrosa, Fabio ; De Souza, Emanuel Maltempi; Monteiro, Rose Adele. Maize Root Lectins Mediate the Interaction with Herbaspirillum seropedicae via N-Acetyl Glucosamine Residues of Lipopolysaccharides. Plos One , v. 8, p. e77001, 2013.

2. Monteiro, Rose A. ; Balsanelli, Eduardo ; Tuleski, Thalita ; Faoro, Helison ; Cruz, Leonardo M. ; Wassem, Roseli ; Baura, Valter A. ; TadraSfeir, Michelle Z. ; Weiss, Vinícius ; DaRocha, Wanderson D. ; MullerSantos, Marcelo ; Chubatsu, Leda S. ; Huergo, Luciano F. ; Pedrosa, Fábio O. ; Souza, Emanuel M. Genomic comparison of the endophyte Herbaspirillum seropedicae SmR1 and the phytopathogen Herbaspirillum rubrisubalbicans M1 by Suppressive Subtractive Hybridization and partial genome sequencing. FEMS Microbiology, Ecology (Print), v. 2012, p. n/an/a, 2012. ;

3. TadraSfeir, M. Z. ; Souza, E. M. ; Faoro, H. ; Muller-Santos, M. ; Baura, V. A. ; Tuleski, T. R.; Rigo, L. U. ; YATES, M. G. ; Wassem, R. ; Pedrosa, F. O. ; Monteiro, R. A. Naringenin Regulates Expression of Genes Involved in Cell Wall Synthesis in Herbaspirillum seropedicae. Applied and Environmental Microbiology (Print), v. 77, p. 21802183, 2011.

Summary published in proceedings of conferences

1. Tuleski, T. R. ; Balsanelli, E. ; Souza, E. M. ; Pedrosa, F. O. ; Monteiro, R. A. Involvement of cellulose biosynthesis genes in biofilm formation by the bacteria Herbaspirillum rubrisubalbicans M1. Tenerife, 2014. 11th European Nitrogen Fixation Conference, 2014.

2. Tuleski, T. R. ; Balsanelli, E. ; Faoro, H. ; Cruz, Leonardo M. ; Wassem, R. ; Baura, V. A. ; Tadra-Sfeir, M. Z. ; Yates, M. G.; Pedrosa, F. O. ; Souza, E. M. ; Monteiro, Rose A. Herbaspirillum rubrisubalbicans M1 Cellulose Biosynthesis gene cluster. Foz do Iguaçu, PR, 2012.

3. Balsanelli, E. ; Tuleski, T. R. ; Faoro, H. ; Cruz, L.M. ; Baura, V. A. ; TadraSfeir, M. Z. ; Wassem, R. ; Chubatsu, L. ; Yates, M. G.; Pedrosa, F. O. ; Souza, E. M. ; Monteiro, R. A. Genomic Comparison of the Endophyte Herbaspirillum seropedicae SMR1 and the phytopathogen Herbaspirillum rubrisubalbicans M1 by SSH and partial genome sequencing. Foz do Iguaçu, PR, 2011. XL Annual Meeting of SBBq, 2011.

4. Tuleski, T. R. ; Monteiro, R. A. ; Balsanelli, E. ; Faoro, H. ; YATES, M. G. ; SFEIR, M. Z. T. ; Baura, V. ; Souza, E. M. ; Pedrosa, F. O. Identification of genetic differences between Herbaspirillum seropedicae and Herbaspirillum rubrisulbalbicans using suppressive subtractive hybridization libraries. Águas de Lindóia, SP, 2009. XXXVIII Annual Meeting of SBBq, 2009.
5. Tuleski, T. R. ; Monteiro, R. A. ; Balsanelli, E. ; Faoro, H. ; SFEIR, M. Z. T. ; Baura, V. ; YATES, M. G. ; Souza, E. M. ; Pedrosa, F. O. Identification of genetic differences between Herbaspirillum seropedicae and Herbaspirillum rubrisulbalbicans using suppressive subtractive hybridization libraries. Curitiba, PR, 2009. 1 International INCT Symposium on Biological Nitrogen Fixation, 2009.

6. Tuleski, T. R. ; Souza, E. M. ; Monteiro, R. A. ; Balsanelli, E. ; Baura, V. ; Faoro, H. ; SFEIR, M. Z. T. Construção e Sequenciamento de Bibliotecas Subtrativas do Gênero Herbaspirillum. In: 17º Evento de Iniciação Científica – UFPR, 2009, Curitiba. A, 2009. Event Classification: Local; Brasil/ Português;

7. Tuleski, T. R. ; Souza, E. M. ; Monteiro, R. A. ; Balsanelli, E. ; Faoro, H. ; Tadra, M. Z. ; Baura, V. ; Pedrosa, F. O. Construção e Sequenciamento de Bibliotecas Subtrativas do Gênero Herbaspirillum. In: 16º Evinci Evento de Iniciação Científica da UFPR, 2008, Curitiba. 16º Evento de Iniciação Científica da UFPR, 2008. Event Classification: Local; Brasil/ Português;

8. Balsanelli, E. ; Souza, Emanuel M. ; Faoro, H. ; Tadra, M. Z. ; Tuleski, T. R. ; Wassem, R. ; Baura, V. A. ; Pedrosa, F. O. ; Monteiro, R.A. Identification of genetic differences between two species of Herbaspirillum. Águas de Lindóia, SP, 2008. XXXVII Annual Meeting of SBBq, 2008.

CONTACT INFORMATION:

Tomas Pellizzaro Pereira (Full CV)

Visiting Ph.D. Student at the Stacey Lab

201 Life Sciences Center

1201 Rollins Road

University of Missouri

Columbia, MO 65211

Email: pellizzaropereirat@missouri.edu

Lab phone No.: 573-884-4799

EDUCATION:

2014-current: PhD in Plant Genetic Resources, Federal University of Santa Catarina, UFSC, Florianopolis, Brazil.
– Title: Interaction between the plant growth promoting bacteria Herbaspirillum seropedicae strain SmR1 and grass plants maize (Zea mays) and Setaria viridis
– Advisor: Ana Carolina Maisonnave Arisi
– Scholarship from: Higher Education Personnel Improvement Coordination – CAPES

2012-2014: Master’s in Plant Genetic Resources. Federal University of Santa Catarina, UFSC, Florianopolis, Brazil.
– Title: Interaction between the plant growth promoting bacteria Herbaspirillum seropedicae strain SmR1 and maize plants: bacterial DNA quantification by qPCR, Year of degree: 2014
– Advisor: Ana Carolina Maisonnave Arisi
– Scholarship from : Higher Education Personnel Improvement Coordination – CAPES 

2007-2011: Bachelor in Agronomy. State University of Santa Catarina, UDESC, Florianopolis, Brazil
– Title: Seeds Quality Control.
– Advisor: Luis Sangoi

PROFESSIONAL EXPERIENCES:

2012-Current: Graduate student , Position: Scholar CAPES , Working hours (weekly): 40, Schemes of job: Full-time and exclusiveness. Federal University of Santa Catarina – UFSC.

2011: Internship , Position: Intern , Working hours (weekly): 40, Schemes of job: Full-time and exclusiveness. Dow AgroSciences – DOWAGRO

2010 & 2011: Scientific Initiation Scholarship, Position: Scholarship FAPESC, Working hours (weekly): 20, Schemes of job: Full-time and exclusiveness. Universidade do Estado de Santa Catarina – UDESC

2008-2009: Scientific Initiation Scholarship, Position: Scholarship FAPESC, Working hours (weekly): 20, Schemes of job: Full-time and exclusiveness. Universidade do Estado de Santa Catarina – UDESC

PUBLICATIONS:

ILHA, EUNICE C.; SCARIOT, MIRELLA C.; TREML, DIANA; PEREIRA, TOMAS P.; SANT’ANNA,
ERNANI S.; PRUDÊNCIO, ELANE S.; ARISI, ANA C. M. Comparison of real time PCR assay and plate count for Lactobacillus paracasei enumeration in yoghurt. Annals of Microbiology, v.66, p.597 606, 2015.

PEREIRA, TOMÁS PELLIZZARO; AMARAL, FERNANDA PLUCANI; DALL’ASTA, PAMELA; BROD, FÁBIO CRISTIANO ANGONESI; ARISI, ANA CAROLINA MAISONNAVE. Real Time PCR Quantification of the Plant Growth Promoting Bacteria Herbaspirillum seropedicae Strain SmR1 in Maize Roots. Molecular Biotechnology, v.56, p.660 670, 2014.

FALEIRO, ALEXANDRO CÉZAR; PEREIRA, TOMÁS PELLIZZARO; ESPINDULA, ELIANDRO; BROD, FÁBIO CRISTIANO ANGONESI; ARISI, ANA CAROLINA MAISONNAVE. Real time PCR detection targeting nifA gene of plant growth promoting bacteria Azospirillum brasilense strain FP2 in maize roots. Symbiosis (Philadelphia, Pa.), v.61, p.125 133, 2013.

ALMEIDA, C. B; ARRUDA, B; PEREIRA, T. P.; VALE, N. M.; HEIDEMANN, J.C; COIMBRA, J. L. M.;
GUIDOLIN, A. F. Is there variability for the character of beans cooking time? Depends of the error! Bioscience Journal (UFU. Impresso), v.27, p.915 923, 2011.

COAN, M. M. D.; STÄHELIN, D.; COIMBRA, J. L. M.; RAFAELI NETO, S. L.; GUIDOLIN, A. F.; PEREIRA,
T. P. Management system of an active genebank of common bean. Crop Breeding and Applied Biotechnology (Impresso), v.10, p.55 60, 2010.

VALE, N. M.; BARILI, L.D.; ROCHA, F.; ROZZETTO, D.S.; PEREIRA, T. P.; COIMBRA, J. L. M.;
GUIDOLIN, A. F; BERTOLDO, J.G. Sowing method a criterion for phenotypic evaluation in bean improvement. Biotemas (UFSC), v.22, p.73 81, 2009.