Laurent Brechenmacher, Ph.D. - Research Scientist
Contact information

 

201 Life Science Center
1201 Rollins Road
University of Missouri
Columbia, MO 65211
Email: brechenmacherl at missouri.edu
Lab: (573) 884 4799

 

 

Education

-Ph D in Biochemistry and Molecular Biology: Investigation of the functional genome involved in the endomycorrhizal symbiosis in barrel medic (Medicago truncatula), National Institute of Agronomic Research/ University of Burgundy, Dijon, France (2003)

 

-MS in Biochemistry and Molecular Biology: Identification of plant genes differentially regulated during the colonisation of M. truncatula by Glomus intraradices using differential screening of cDNA libraries, University of Burgundy, Dijon, France (1999)

 

-BS in Biochemistry and Molecular Biology: University of Franche Comté, Besançon, France (1998)

 

 

Professional Experiences
-Post-doctoral fellow: Analysis of soybean root hair infection by the symbiotic bacteria Bradyrhizobium japonicum using transcriptomic and proteomic approaches, National Center for Soybean Biotechnology, University of Missouri-Columbia, USA (January 2005-present)

 

-Post-doctoral fellow: 1) Evaluation of the function of M. truncatula genes in the establishment and functioning of the mycorrhizal symbiosis using TILLING approach, National Institute of Agronomic Research/ University of Burgundy, Dijon, France (December 2003-November 2004)
2) Teaching of Biochemistry at the University of Burgundy (December 2003-November 2004)

 

-Graduate student: Identification of plant genes differentially expressed in a well established symbiosis between M. truncatula and G. mosseae using suppression subtractive hybridisation and DNA microarray approaches, National Institute of Agronomic Research/ University of Burgundy, Dijon, France (October 1999-November 2003)

 

-Undergraduate student: 1) Identification of plant genes differentially expressed in a well established symbiosis between M. truncatula and G. intraradices using differential screening of cDNA libraries (Phytoparasitology laboratory, National Institute of Agronomic research, Dijon, France (July 1998-September 1999)
2) Development of two-dimensional electrophoretic techniques for paternity identification tests, Blood centre, Besançon, France (July-August 1997)

 

 

Current Research Focus

 

 

Proteomic and metabolomic analysis of Soybean Root Hairs Colonized by Bradyrhizobium japonicum. Proteomic analysis of Soybean NIL seed filling.

 

 

Publications

 

Journal Articles
Gollotte, A., Brechenmacher, L., Weidmann, S., Franken, P., and Gianinazzi-Pearson, V. 2002. Plant genes involved in arbuscular mycorrhiza formation and functioning. In: Gianinazzi, S., Schüepp, H., Barea, J.M., and Haselwandter, K. (eds) Mycorrhizal technology in agriculture: from genes to bioproducts. Birkhäuser publisher. pp 87-102.
Gianinazzi-Pearson, V., Brechenmacher, L., Weidmann, S., Sanchez, L., Gianinazzi, S., and van Tuinen, D. (2004). Molecular dialogue in arbuscular mycorrhiza: getting to the symbiotic cell programme. Proc.11 th Intern. Congress on Molecular Plant-Microbe Interactions. Eds.

 

Tikhonovich I.A., Lugtenberg B.J.J., Provorov N.A. IS-MPMI. St.-Petersburg, Russia.
Sanchez, L., Weidmann, S., Brechenmacher, L., Batoux, M., van Tuinen, D., Lemanceau, P., Gianinazzi, S., and Gianinazzi-Pearson, V. (2004). Common gene expression in Medicago truncatula roots in response to Pseudomonas fluorescens colonisation, mycorrhiza development and nodulation. New Phytologist. 161: 855-863.
Küster, H., Hohnjec, N., Krajinski, F., Yahyaoui, F.E., Manthey, K., Gouzy, J., Dondrup, M., Meyer, F., Kalinowski, J., Brechenmacher, L., van Tuinen, D., Gianinazzi-Pearson, V., Pühler, A., Gamas, P., and Becker, A. (2004). Construction and validation of comprehensive cDNA-based macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula. Journal of Biotechnology. 108: 95-113.
Brechenmacher, L., Weidmann, S., van Tuinen, D., Chatagnier, O., Gianinazzi, S., Franken, P., and Gianinazzi-Pearson, V. (2004). Expression profiling of up-regulated plant and fungal genes in early and late stages of Medicago truncatula/Glomus mosseae interactions. Mycorrhiza. 14: 253-262.
Gianinazzi-Pearson, V. and Brechenmacher, L. (2004). Functional genomics of arbuscular mycorrhiza: decoding the symbiotic cell programme. Canadian Journal of Botany. 82: 1228-1234.
Stacey, G., Libault, M., Brechenmacher, L., Wan, J., and May, G. D. (2006). Genetics and functional genomics of legume nodulation. Current Opinion in Plant Biology. 9:110-121.
Massoumou, M., van Tuinen, D., Chatagnier, O., Arnould, C., Brechenmacher, L., Sanchez, L., Selim, S., Gianinazzi, S., Gianinazzi-Pearson, V. (2007). Medicago truncatula gene responses specific to arbuscular mycorrhiza interactions with different species and genera of Glomeromycota. Mycorrhiza. 17: 223-234.
Song, Z., Chen, L., Ganapathy, A., Wan, X.F., Brechenmacher ,L., Tao, N., Emerich, D., Stacey, G., and Xu. D. (2007). Development and assessment of scoring functions for protein identification using PMF data. Electrophoresis. 28: 864-870.
Brechenmacher, L., Kim, M.Y, Benitez, M., Li, M., Joshi, T., Calla, B., Lee, M.P., Libault, M., Vodkin, L.O., Xu, D., Lee, S.H., Clough, S.J., and Stacey, G. (2008). Transcription profiling of soybean nodulation by Bradyrhizobium japonicum. Molecular Plant-Microbe Interactions. 21: 631-645.
Brechenmacher, L., Lee, J., Sachdev, S., Song, Z., Nguyen, T.H.N., Joshi, T., Oehrle, N., Libault, M., Mooney, B., Xu, D., Cooper, B., and Stacey, G. (2009) Establishment of a protein reference map for soybean root hair cells. Plant Physiology. 149: 670-682.

 

Selected communications
Brechenmacher L., Sachdev S., Dague B., Lee J., Oehrle N., Libault M., Mooney B., Cooper B., Stacey G. Establishment of the soybean root hair proteome reference map. The 11th Biennial conference on the Molecular and Cellular Biology of the Soybean, Lincoln, USA, 5-8 August 2006 (poster).
Brechenmacher L., Kim M.Y., Govindarajulu M., Benitez M., Li M., Lee M.P., Libault M., Lee S.H., Taylor C., Clough S.J., Stacey G. Transcript Profiling of Soybean during Nitrogen Fixation Symbiosis. The 11th Biennial conference on the Molecular and Cellular Biology of the Soybean, Lincoln, USA, 5-8 August 2006 (poster).
Brechenmacher L., Sachdev S., Dague B., Lee J., Oehrle N., Libault M., Mooney B., Cooper B., Stacey G.  Proteome Reference Map of Soybean Root Hair. Plant Protein Phosphorylation Dephosphorylation, Columbia, USA, 22-24 May 2007 (poster).
Brechenmacher L., Sachdev S., Dague B., Lee J., Oehrle N., Libault M., Mooney B., Cooper B., Stacey G.  Proteome Reference Map of Soybean Root Hair. Plant Proteomics Mini-Symposium, Columbia, USA, 24-25 May 2007 (poster).
Brechenmacher L., Kim M. Y., Govindarajulu M., Benitez M., Li M., Lee M. P., Libault M., Lee S. H., Taylor C., Clough S.J., Stacey G. Transcript Profiling of Soybean During The Nitrogen Fixation Symbiosis. The 20th North American Symbiotic Nitrogen Fixation Conference, Milwaukee, USA, 10-14 July 2007 (poster).
Brechenmacher L., Sachdev S., Dague B., Lee J., Oehrle N., Libault M., Mooney B., Cooper B., Stacey G.  Soybean Root Hair Proteome Reference Map. Heartland Mass Spectrometry Workshop, Biological applications, Osage Beach, USA, 26-26 October 2007 (poster).
Brechenmacher L., Sachdev S., Dague B., Lee J., Oehrle N., Libault M., Mooney B., Cooper B., Stacey G. Establishment of a protein reference map of soybean root hair cell by 2D gel electrophoresis and MudPIT. The 12th Biennial conference on the Molecular and Cellular Biology of the Soybean, Indianapolis, USA, 20-23 July 2008 (poster).
Brechenmacher L., Lei,Z.T., Hixson K., Libault M., Findley S., Lowery R., Mooney B., Pasa Tolic L., Sumner L.W., Stacey G. Proteomic and metabolomic analysis of Soybean Root Hairs Colonized by Bradyrhizobium japonicum. The 16th International Congress on Nitrogen Fixation, Big Sky, USA, 14-19 June 2009 (poster).