NGUYEN VAN MANH, M.Sc.
- FIELDS OF SPECIALISATION
Plant Molecular Biology, Plant Breeding, Transformation
- EDUCATION:
Can Tho University, College of Agriculture
May 2020
Bachelor of Science, Plant Breeding Engineering
May 2022
Master of Science, Genetics and Plant Breeding
- PROFESSIONAL EXPERIENCE:
- 2018 – 2022:
Can Tho University – Ninh Kieu District, Cantho Province, Vietnam
Research assistant: Performed molecular biology and microbiology techniques at the laboratory of Genetic and Plant Breeding, College of Agriculture, Can Tho University: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Polyacrylamide Gel Electrophoresis, cDNA Library Synthesis, rice tissue culture, rice transformation, and ornamental tissue culture. In addition, some antioxidant activities (Anthocyanins, Flavonoids, and Polyphenols) and secondary compounds such as ABTS (2,22-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid), DPPH (1,1-Diphenyl-2-picrylhydrazyl). Along with that Bioinformatic tools like Rstudio, Origin, and SPSS.
- February 2020 – August 2020
Institute of Biotechnology, Vietnam Academy of Science and Technology – Cau Giay District, Hanoi, Vietnam
CRISPR/Cas9 technology, rice transformation with Doctor Do Tien Phat
- May 2022 – Now:
Bing Stacey Lab, University of Missouri – Columbia, USA:
I am currently employed as a research fellow at the Christopher S. Bond Life Science Center within the Division of Plant Science and Technology at the University of Missouri-Columbia, USA. My research primarily aims to identify novel genes involved in the regulation of protein, oil, and sugar content in soybeans using fast neutron (FN) mutagenesis through a forward genetics approach. Additionally, I am developing multiplex CRISPR/Cas9 gene editing technology to further understand these genes molecular and physiological functions via reverse genetics. Furthermore, our research seeks to dissect the robust gene regulatory network that underlies proteome rebalancing in soybean seeds, which can tightly control seed protein content and amino acid composition, even after major genetic perturbation of seed storage proteins (SSPs).
Furthermore, my research focuses on SAMS mutants (S-adenosylmethionine synthetase) in soybean, which is synthesized from methionine and adenosine triphosphate (ATP) by the enzyme S-adenosylmethionine synthetase (SAMS). Using CRISPR-Cas9, we generated soybean SAMS mutants to investigate whether enhancing the methionine and other amino acid content in developing and mature seeds is possible by targeting the metabolism of the S-adenosylmethionine (SAMS) gene.
The most important agronomic trait targeted for soybean crop improvement is increased yield, which can be achieved by enhancing seed number and/or seed weight. Phenotypic screening of fast neutron-induced soybean mutants identified a mutant line with a higher proportion of four-seeded pods (4-SP), producing approximately 30% 4-SP compared to ~4% in the wild type. Genetic mapping and co-segregation analyses revealed that the 4-SP phenotype in the mutant is controlled by the additive effects of at least two alleles. Furthermore, translational studies on knock-out mutants of orthologous genes in Arabidopsis and Tomato showed increased locule production in siliques and fruits, respectively, suggesting functional conservation across plant species.
- TRAINING & CERTIFICATIONS
December 2018: College of Agriculture, Can Tho University
Course: “Phenotypic and Genetic analysis for salinity stress response in rice”
February 2020 – August 2020: Institute of Biotechnology (IBT)
Course: “CRISPR/CAS9 technology, and rice transformation”
April 2021: College of Agriculture, Can Tho University
Course: “Applied bioinformatics in rice breeding”
- PUBLICATIONS
BOOKS
Ky, H., Phat, D.T., Manh, N.V., Linh, N.T., & Do, T.I. (2021). Genetic engineering through agrobacterium tumefaciens in rice indica. Cantho University Publishers.
Ky, H., Tam, N.T., Manh, N.V., Giang, V.Q., Do, T.I., & Khang, C.T.Q. (2021). Analysis of rice quality traits by molecular and biochemical methods. Cantho University Publishers.
Ky, H., Tung, N.C.T., Hien, N.L., Dien, H.N., Tu, P.T.B., Thanh, L.T.H., Manh, N.V., Giang, V.Q., Do, T.I., & Khang, C.T.Q. (2021). Functional gene markers in rice breeding. Cantho University Publishers.
JOURNALS
Ky, H., Tung, N.C.T., Hien, N.L., Manh, N.V., Thanh, N.N., Thanh, V.C., & Yeap, S.K. (2023). Novel deletion in exon 7 of Betaine Aldehyde Dehydrogenase 2 (BADH2). Rice Science, 30(2), 104-112.
Phuc, T.H., Giang, V.Q., Manh, N.V., & Ky, H. (2021). Genetic diversity of local rice varieties (Oryza sativa L.) in Vietnam’s Mekong delta based on SSR markers and morphological characters. Indonesian Journal of Biotechnology, 26(2), 2021, 76‐81
Manh, N.V., Do, T.P., To, T.T.T., Nguyen, V.T., & Huynh, K. (2023). Agrobacterium-mediated transformation of Nang Thom Cho Dao, an indica rice variety. CTU Journal of Innovation and Sustainable Development, 15(2), 51-59.
Manh, N.V., Dien, H.N., Giang, V.Q., Tung, N.C.T., Hien, N.L., Thanh, L.T.H., & Ky, H. (2020). Evaluation for salt tolerance genotype of 40 improved rice accessions using SSR markers. Can Tho University Journal of Science, 56: 102-108
Manh, N.V., Phat, D.T., Do, T.I & Ky, H. (2021). The effective method for regeneration in rice tissue culture of Nang Thom Cho Dao variety. Can Tho University Journal of Science, 56
Manh, N.V., Do, T.I., Giang, V.Q., Dien, H.N., Thanh, L.T.H., & Ky, H. (2021). Application of SSRs markers for selection of salt tolerance and quality genotype in 20 improvement rice varieties/lines. Can Tho University Journal of Science, 57 (3), 147-156
Giang, V.Q., Do, T.I., Manh, N.V., Tam, N.T., Dien, H.N., Thanh, L.T.H., & Ky, H. (2021). The variation of OsHKT family gen in 41 Mekong delta rice varieties. Can Tho University Journal of Science, 57 (6), 224-230
Khang, C.T.Q., Dien, H.N., Thanh, L.T.H., Hien, N.L., Manh, N.V., Do, T.I., Tu, P.T.B., Tung, N.C.T., & Ky, H. (2021). Phenotypic characterization of 147 upland rice. Can Tho University Journal of Science, 57, 56B
Ky, H., Giang, V.Q., Manh, N.V., Do, T.I., Tam, N.T., Tung, N.C.T., & Hien, N.L. (2021). Differential gene expression under salt stress at seedling stage as a response of Doc Phung and nep Mo varieties. Can Tho University Journal of Science, 57(3), 114-121.
Ky, H., Hien, N.L., Giang, V.Q., Manh, N.V., Khang, C.T.Q., Do, T.I., & Tung, N.C.T. (2021). Genetic Diversity Of 120 Soybean (Glycine Max (L.) Merr.) Varieties/Lines Using SSR Markers. Journal of Agricultural Science and Technology of the University of Agriculture and Forestry, Hue, 5(3), 2606-2613.
Ky, H., Manh, N.V., Thoang, D.T.T., Duy, N.K., Do, T.I., Khang, C.T.Q., … & Hien, N.L. (2023). Agro-morphological and genotypic characteristics of 29 upland rice varieties cultivated in the Mekong Delta. Can Tho University Journal of Science, 59(5), 161-174.
Ky, H., Phat, T.D.T., Phung, N.T.K., Giang, V.Q., Manh, N.V., Do, T.I., … & Dien, H.N. (2021). Variation of OsTZF1 gene related to salt tolerance in Doc Phung rice variety using whole genome sequencing. Can Tho University Journal of Science, 57(4), 159-168.
Ky, H., Thep, N.V., Giang, V.Q., Manh, N.V., Do, T.I., Dien, H.N., … & Tu, P.T.B. (2021). The effectiveness of functional markers for evaluation of rice quality traits. Can Tho University Journal of Science, 57(4), 119-130.
Thao, B.P., Linh, N.T., Manh, N.V., Linh, L.K., Ha, C.H., Phat, D.T., & Ngoc, P.B. (2021). Optimization of Agrobacterium-mediated transformation procedure for an indica rice variety Khangdan 18. Journal of Biotechnology, 20 (1), 53-62
Proceeding
- CONFERENCES
Soy2023 Biennial International Conference, University of Nebraska – Lincoln, USA, Aug.10-13, 2023. The poster 3rd place.
- AWARD
August 2021
Receive a Vallet scholarship with a value of 20 million VND